Hobaneng ha lisebelisoa tsa PCR hangata li etsoa ka PP?

"Joalokaha bohle re tseba, PCR ke mokhoa oa motheo oa liteko lilaboratoring tsa biochemical." Liphetho tsa liteko li lula li sa khotsofatse, tse ka 'nang tsa bakoa ke tšilafalo e fokolang ea lisebelisoa tsa polasetiki tsa PCR, kapa tšitiso ea liteko e bakoang ke ho kenngoa ha li-inhibitors. Ho na le lebaka le leng la bohlokoa haholo: Khetho e fosahetseng ea lisebelisoa le eona e tla ba le tšusumetso e kholo liphethong tsa liteko.

Ho na le mabaka a mangata a amang liphetho tsa liteko tsa PCR: hangata ho na le mefuta e 7 e latelang.

1. Li-Primers: Li-primers ke senotlolo sa karabelo e khethehileng ea PCR, 'me ho khetheha ha lihlahisoa tsa PCR ho itšetlehile ka tekanyo ea ho tlatsana pakeng tsa li-primers le DNA ea template;

2. Enzyme le mahloriso a eona;

3. Boleng le mahloriso a dNTP;

4. Template (lefu la sepheo) nucleic acid;

5. Mg2 + mahloriso;

6. Ho beha mocheso le nako;

7. Palo ea lipotoloho;

8. Thepa, lisebelisoa, joalo-joalo.

Har'a lintho tse ngata tse susumetsang, lisebelisoa ke e 'ngoe ea lintlha tsa bohlokoa haholo le tse hlokomolohuoang habonolo.

Ho na le mefuta e mengata eaLisebelisoa tsa PCR: Li-tubes tse 8, li-tubes tse tlaase, li-tubes tse tloaelehileng, tse se nang seaparo, li-semi-skirted, li-skirted tse feletseng, le letoto la lipoleiti tsa PCR le qPCR. Ho thata haholo ho khetha, 'me ho na le mathata a mangata a Tloaelehileng, ha re shebeng mathata ao bohle ba a khethangLisebelisoa tsa PCR, le mokhoa oa ho li rarolla?

Hobaneng baLisebelisoa tsa PCRhangata e entsoe ka PP?

Karabo: Lisebelisoa tsa PCR / qPCR ka kakaretso li entsoe ka polypropylene (PP), hobane ke ntho e sa sebetseng ea biologically, holimo ha ho bonolo ho khomarela li-biomolecules, 'me e na le lik'hemik'hale tse ntle tsa lik'hemik'hale le mamello ea mocheso (e ka ba autoclaved ka likhato tse 121) libaktheria. hape e ka mamella liphetoho tsa mocheso nakong ea ho palama baesekele e futhumetseng). Lisebelisoa tsena hangata li amana ka ho toba le li-reagents kapa lisampole, kahoo lisebelisoa tsa boleng bo phahameng le mekhoa e metle ea ho sebetsa li lokela ho khethoa nakong ea tlhahiso le ho lokisetsa.