Lehae > Blog > Litaba tsa indasteri

Letlapa la PCR ke eng

2023-03-18

TheLetlapa la PCRke sesebelisoa se sebelisoang haholo e le li-primers, dNTPs, buffers, joalo-joalo se amehang karabelong ea amplification ho polymerase chain reaction. TheLetlapa la PCRe hlahisoa ka bio-polypropylene ea boleng bo holimo sebakeng sa tlhahiso e hloekileng haholo, ho etsoa ha hlobo e phahameng ka ho fetesisa le mokhoa o nepahetseng oa ho bopa polasetiki ho netefatsa boleng ba sehlahisoa le ho ts'oana le botsitso ba lihlahisoa lipakeng tsa lihlopha.

Likaroloana:

1. Lerako la tube le tšesaane, botenya ba lerako bo tšoana, bokhoni ba ho fetisa mocheso bo potlakile, 'me sampuli e futhumetse ka ho lekana.

2. E ka etsoa sterilized ka mocheso o phahameng le khatello e phahameng.

3. Litlhaku, linomoro le mela ea ho tšoaea li ngotsoe ka pele bakeng sa ho tsebahatsa kapele le ho khetholla lisampole.

4. E loketse karabelo ea PCR mme e ka sebelisoa ka li-caps tse robeli kapa li-caps tse leshome le metso e 'meli.


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