Kenyelletso ea Elisa Plate

ELISA PLATE: Ho Enzyme Linked Immunosorbent Assay (ELISA), bohloeki, mahloriso le karo-karolelano ea li-antigens, li-antibodies, li-antibodies tse ngotsoeng kapa li-antigen tse amehang karabelong ea immunology; mofuta oa buffer, mahloriso le Maemo a kang matla a ionic, boleng ba pH, mocheso oa karabelo le nako li bapala karolo ea bohlokoa. Ho phaella moo, holim'a polystyrene e tiileng-phase (Polystyrene) e le mojari e boetse e phetha karolo ea bohlokoa ho adsorption ea li-antigen, li-antibodies kapa li-antigen-antibody complexes.

Li-antigen, li-antibodies le li-biomolecules tse ling li khangoa sebakeng se tsamaisang thepa ka mekhoa e fapaneng, ho kenyelletsa le adsorption e sa sebetseng ka li-bond tsa hydrophobic, hydrophobic / ionic bond, tlamahano e kopanetsoeng ka ho hlahisoa ha lihlopha tse ling tse sebetsang joalo ka lihlopha tsa amino le carbon, le ka ho fetola bokaholimo. . Hydrophilic bonding ka mor'a thobalano.

TheElisa Platee ka aroloa ka 48-seliba le 96-hantle ho ea ka palo ea masoba. E sebelisoang hangata ke 96-well, e lokelang ho khethoa ho latela 'mali oa hau oa microplate.

Ho phaella moo, ho na le tse ka tlosoang le tse sa khoneng ho tlosoa. Bakeng sa tse sa khoneng ho tlosoa, li-slats holim'a boto eohle li kopantsoe hammoho. Joale, bakeng sa tse senyehang, li-slats ka boto li arotsoe, 'me mapolanka a arohaneng Ho na le lihlopha tse 12 le likoti tse 8. Ka kakaretso, lipoleiti tse ngotsoeng ka li-enzyme tse senyehang li sebelisoa hangata kajeno. Haeba u kile ua reka lipoleiti tse joalo pele, u ka reka feela likhoele tse ngotsoeng ka enzyme hona joale.

Le hoja li-microplates tse entsoeng ke bahlahisi ba fapaneng li shebahala li tšoana ka kakaretso, lintlha tse ling tse nyenyane li tla fapana, tse kang sebopeho, joalo-joalo Sena ke haholo-holo hobane se hloka ho sebelisoa le babali ba microplate ba fapaneng. Ka hona, ha u sebelisa Ha u khetha ho reka sebali sa microplate, u lokela hape ho nahana ka hore na 'mali oa hau oa microplate o shebahala joang. Empa ka kakaretso li ikamahanya le maemo, ke tse ling feela tse tla fapana. Hobane thepa ea poleiti ea enzyme hangata ke polystyrene (PS), 'me polystyrene e na le botsitso bo fokolang ba lik'hemik'hale' me e ka qhibiliha ka mefuta e fapaneng ea lihlapolli (tse kang li-hydrocarbon tse nkhang hamonate, li-halogenated hydrocarbons, joalo-joalo), 'me li tla chesoa ke li-acid tse matla. le alkalis. , ha e hanyetsane le mafura, 'me ho bonolo ho fetola' mala ka mor'a ho pepesehela leseli la ultraviolet, kahoo e-ba bonnete ba hore u ela hloko tsena ha u sebelisaElisa Plate.