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Mesebetsi ea ELISA kit ke efe?
2022-12-23
ELISA Kit e ipapisitse le karolo e tiileng ea antigen kapa antibody le ho ngolisoa ha enzyme ea antigen kapa antibody. Antigen kapa antibody e tlameletsoeng ka holim'a sejari se tiileng e ntse e boloka tšebetso ea eona ea sesole sa 'mele, 'me enzyme e bitsoang antigen kapa antibody e boloka tšebetso ea eona ea ho itšireletsa mafung le tšebetso ea enzyme. Nakong ea qeto, mohlala o tlas'a teko (moo ho lekanngoang antigen kapa antigen) o sebetsana le antigen kapa anti-antibody e holim'a mochine o tiileng. Antigen-antibody complex e entsoeng holim'a sejari se tiileng e arohanngoa le lintho tse ling tse mokelikeli ka ho hlatsoa.
Li-antigen kapa li-antigen tse ngotsoeng ke enzyme lia eketsoa, tse boetse li tlamella sejari se tiileng ka karabelo. Ka nako ena, palo ea enzyme karolong e tiileng e lekana le bongata ba ntho e hlahang setšoantšong. Kamora ho kenyelletsa karoloana ea karabelo ea enzyme, substrate e hlohlelletsoa ke enzyme hore e fetohe lihlahisoa tse mebala. Palo ea sehlahisoa e amana ka kotloloho le bongata ba ntho e lekiloeng ka har'a mohlala, kahoo tlhahlobo ea boleng kapa palo e ka etsoa ho latela botebo ba 'mala.
Ts'ebetso e phahameng ea matla ea li-enzyme ka tsela e sa tobang e holisa litholoana tsa karabelo ea 'mele ea ho itšireletsa mafung, e leng se etsang hore tlhahlobo e be bonolo haholo. ELISA e ka sebelisoa ho fumana li-antigens, empa hape e ka sebelisoa ho fumana li-antibodies.
Melao-motheo ea motheo ea ELISA kit
E sebelisa karabelo e itseng ea antigen le antibody ho hokahanya ntho le enzyme, ebe e hlahisa karabelo ea mebala lipakeng tsa enzyme le substrate bakeng sa boikemisetso ba bongata. Ntho ea ho metha e ka ba antibody kapa antigen.
Ho na le li-reagents tse tharo tse hlokahalang ka mokhoa ona oa boikemisetso:
â Antigen ea mohato o tiileng kapa sethibela-mafu (immune adsorbent)
â¡ Enzyme e ngotsoeng antigen kapa antibody (lesupa)
⢠substrate bakeng sa ts'ebetso ea enzyme (setsi sa nts'etsopele ea mebala)
Tekanyong, antigen (antigen) e qala ho tlamelloa ho mojari o tiileng, empa e ntse e boloka ts'ebetso ea eona ea 'mele ea ho itšireletsa mafung, ebe ho kenyelletsoa conjugate (letšoao) la antibody (antigen) le enzyme, e ntseng e boloka ts'ebetso ea eona ea pele ea ho itšireletsa mafung le enzyme. mosebetsi. Ha conjugate e sebetsana le antigen (antibody) ho sejari se tiileng, substrate e tsamaellanang ea enzyme ea eketsoa. Ke hore, catalytic hydrolysis kapa REDOX karabelo le mmala.
Li-antigen kapa li-antigen tse ngotsoeng ke enzyme lia eketsoa, tse boetse li tlamella sejari se tiileng ka karabelo. Ka nako ena, palo ea enzyme karolong e tiileng e lekana le bongata ba ntho e hlahang setšoantšong. Kamora ho kenyelletsa karoloana ea karabelo ea enzyme, substrate e hlohlelletsoa ke enzyme hore e fetohe lihlahisoa tse mebala. Palo ea sehlahisoa e amana ka kotloloho le bongata ba ntho e lekiloeng ka har'a mohlala, kahoo tlhahlobo ea boleng kapa palo e ka etsoa ho latela botebo ba 'mala.
Ts'ebetso e phahameng ea matla ea li-enzyme ka tsela e sa tobang e holisa litholoana tsa karabelo ea 'mele ea ho itšireletsa mafung, e leng se etsang hore tlhahlobo e be bonolo haholo. ELISA e ka sebelisoa ho fumana li-antigens, empa hape e ka sebelisoa ho fumana li-antibodies.
Melao-motheo ea motheo ea ELISA kit
E sebelisa karabelo e itseng ea antigen le antibody ho hokahanya ntho le enzyme, ebe e hlahisa karabelo ea mebala lipakeng tsa enzyme le substrate bakeng sa boikemisetso ba bongata. Ntho ea ho metha e ka ba antibody kapa antigen.
Ho na le li-reagents tse tharo tse hlokahalang ka mokhoa ona oa boikemisetso:
â Antigen ea mohato o tiileng kapa sethibela-mafu (immune adsorbent)
â¡ Enzyme e ngotsoeng antigen kapa antibody (lesupa)
⢠substrate bakeng sa ts'ebetso ea enzyme (setsi sa nts'etsopele ea mebala)
Tekanyong, antigen (antigen) e qala ho tlamelloa ho mojari o tiileng, empa e ntse e boloka ts'ebetso ea eona ea 'mele ea ho itšireletsa mafung, ebe ho kenyelletsoa conjugate (letšoao) la antibody (antigen) le enzyme, e ntseng e boloka ts'ebetso ea eona ea pele ea ho itšireletsa mafung le enzyme. mosebetsi. Ha conjugate e sebetsana le antigen (antibody) ho sejari se tiileng, substrate e tsamaellanang ea enzyme ea eketsoa. Ke hore, catalytic hydrolysis kapa REDOX karabelo le mmala.
Moriti oa 'mala oo e o hlahisang o lekana le palo ea antigen (antibody) e lokelang ho lekanyetsoa. Sehlahisoa sena se mebala se ka bonoa ka leihlo le hlobotseng, microscope ea optical, microscope ea elektronike, e ka boela ea lekanyetsoa ka spectrophotometer (sesebelisoa sa label ea enzyme). Mokhoa o bonolo, o bonolo, o potlakile ebile o totobetse.
E fetileng:Kenyelletso ea Elisa Plate
