Tataiso ea Litlhahiso tse fapaneng tsa Laboratori ea Pipette

Litlhahiso tsa pipette ke eng?

Litlhahiso tsa Pipette ke lisebelisoa tse lahloang bakeng sa li-pipette tse sebelisetsoang ho fetisa metsi ka nepo. Li tla ka boholo bo fapaneng, lisebelisoa le mefuta, joalo ka malebela a tloaelehileng, a tlase, a tlhotliloeng le a bolelele bo atolositsoeng.

Malebela a Pipette a bohlokoa lilaboratoring tsa mahlale 'me a sebelisoa haholo ho mahlale a bophelo, k'hemistri, meriana, biotechnology, le biology ea limolek'hule. Ka lebaka la likopo tsa bona tse fapaneng, mekhatlo e laolang lefats'e ka bophara e beha litekanyetso tsa boleng ho netefatsa ho nepahala le ho ts'epahala ha lipatlisiso. Cotaus, moetsi ea tummeng oa lisebelisoa tsa tlhaho tsa tlhaho Chaena, o hlahisa malebela a boleng bo holimo a pipette a netefalitsoeng ke ISO, CE, le FDA, ho netefatsa ho ts'epahala le ho lateloa ha lipatlisiso tsa mahlale.

Kajeno, a re hlahlobeng mefuta e fapaneng ea malebela a pipette, ho utloisisa likarolo tsa bona tse ikhethang le ts'ebeliso ea ts'ebetso e nepahetseng ea mokelikeli.

Mefuta e fapaneng ea malebela a pipette

1. Litlhahiso tsa Pipette tse tloaelehileng (Bokahohle).

Malebela a tloaelehileng a pipette, a tsejoang hape e le malebela a bokahohleng, hangata a entsoe ka polypropylene ea boleng bo holimo, e ikemetseng. Ke mofuta o atileng haholo oa lisebelisoa tsa pipette tse sebelisoang lilaboratoring tse nang le litlhoko tse fapaneng tsa ts'ebetso tse fapaneng ho tloha ho ts'ebetso e phahameng ho isa ho li-reagent dispensing ka mamello e kholo, e etselitsoeng ho lekana mefuta e mengata ea li-pipette le mehlala, e li etsang hore li feto-fetohe ebile li loketse mokelikeli o akaretsang. ho sebetsana le mesebetsi. E fumaneha ka mefuta e 'meli e hloekisitsoeng le e sa tsoakoang ho latela litlhoko tse khethehileng tsa teko.

Malebela a sa Hlapang vs

Malebela a sa Nyokoang:Tsena li ka sebelisoa bakeng sa lits'ebetso tse akaretsang tsa laboratoring moo ho sterility ho seng bohlokoa. Li na le chelete e ngata bakeng sa mesebetsi e tloaelehileng kapa lisampole tse sa tsotelleng.

Malebela a nyopa: Li bohlokoa bakeng sa lits'ebetso tse hlokolosi tse kang microbiology, molecular biology, le liteko tsa bongaka, kaha li entsoe esale pele 'me li netefalitsoe hore ha li na litšila tse joalo ka RNase, DNase, le endotoxins joalo-joalo. Ho ka utloahala ho khahlisa ho kenya malebela a sa hloekang holim'a likokoana-hloko. empa autoclaving e ka 'na ea felisa kotsi ea tšoaetso e bakoang ke lintho tse phelang, sena ha se bolele hore malebela a tla be a se na letho. RNase le DNase.

Haeba o hloka ho etsa liteko tse hlokolosi moo sena se hlokahalang, o lokela ho khetha malebela a sterile pipette ho tsoa ho moetsi ea ka netefatsang hore malebela a bona ha a na RNase le DNase.

Cotausmalebela a tloaelehilengtla ka boholo bo fapaneng ba molumo (mohlala, 10 µL, 20 µL, 50 µL, 100 µL, 200 µL, 300 µL, 1000 µL).

2. Sefa vs Non-FilterTips

Malebela a Sefe:Malebela a tlhotliloeng a na le tšitiso e nyane, eo hangata e entsoeng ka thepa ea hydrophobic, e ka hare ho ntlha. Setlhopha sena se thibela tšilafalo pakeng tsa lisampole le pipette. Malebela a ho sefa hangata a etselitsoe ho sebelisoa mefuteng e itseng ea liteko. Mohlala, haeba sampole e senya, e sa fetohe, kapa e na le viscous haholo ka tlhaho, e ka senya pipette. Maemong a joalo, malebela a filthara a khothaletsoa.

Nako le nako ha u lakatsa metsi, li-aerosol li hlahisoa ka har'a ntlha ea pipette. Haeba u sa sebelise malebela a sefe, li-aerosol tsena li kanna tsa silafatsa pipette ea hau le lisampole tse latelang, tse amang liphetho tsa liteko tsa hau. Ka hona, malebela a li-filters a na le litšenyehelo tse phahameng haholo litekong tse nepahetseng.

Malebela a sa Sefeng:Malebela a sa sefeng ke malebela a sebelisoang hangata ka li-pipette lilaboratoring kaha a theko e tlase ho feta malebela a sefa. Li loketse ka ho fetisisa bakeng sa mehlala e sa tloaelehang ea ho silafala le ho ke keng ha etsahala hore e senye pipette. joalo ka ho arola plasmid DNA, le ho kenya li-gel tsa agarose, har'a tse ling. Leha ho le joalo, ha ba na melemo ea ho thibela tšilafalo ea malebela a sefa, e leng se etsang hore ba se lokele liteko tse mahlonoko kapa tse hlokolosi.

3. Malebela a Poloko a Tlase vs Malebela a sa Poso e Tlase (E Tloaelehileng)

Litlhahiso tse tlaase tsa ho boloka pipetteli etselitsoe ho fokotsa ho bolokoa ha mokelikeli ka hare ho ntlha, ho netefatsa phetisetso e nepahetseng le e sebetsang hantle ea sampole. Malebela ana a nepahetse bakeng sa ho sebetsa ka lino tse tahang, tse khomarelang, kapa tsa bohlokoa moo ho leng bohlokoa ho fokotsa tahlehelo ea sampole. Leha ho le joalo, li na le chelete e ngata ho feta litlhahiso tse tloaelehileng, litlhahiso tsena li loketse ho bokella lisampole nakong ea PCR, ho hloekisoa ha protheine, SDS-PAGE, cloning, DNA le RNA applications hammoho le mefuta e sa tšoaneng ea tlhahlobo ea protheine.

4. Malebela a Khutšoane vs. Bolelele bo Atolositsoeng

Litlhahiso tse khutšoanyane tsa pipetteli etselitsoe ho sebelisoa lipoleiting tse nang le liliba tse ngata, tse kang 1536 kapa liforomo tsa liliba tse 384, moo boholo ba tsona bo fokolang bo thusang ho lebisa liliba tse moqotetsane ka nepo. Malebela ana a boetse a ntlafatsa ergonomics ka ho lumella pipetting haufi le benche, ho fokotsa khatello ea matsoho nakong ea mesebetsi e pheta-phetoang. E loketse bakeng sa tlhahlobo e phahameng ea lihlahisoa le ho ntlafatsa boiketlo ba laboratori.

Malebela a malelele a pipetteli telele ho feta malebela a tloaelehileng, ho fana ka taolo e ntle ea tšilafalo ka ho lumella ho kena ka tlase ho likepe ha ho ntse ho fokotsa ho kopana le setshelo. Malebela ana a loketse ho sebelisoa le lisebelisoa tsa lab joalo ka li-blocks tsa liliba tse tebileng le li-tubes tsa microcentrifuge, tse netefatsang ts'ebetso e nepahetseng ea mokelikeli libakeng tseo ho leng thata ho fihla ho tsona.

5. Malebela a Pipette a pharalletseng

Litlhahiso tse pharalletseng tsa pipettee na le ntlha ea distal e nang le orifice e fihlang ho 70% e kholoanyane ho feta malebela a tloaelehileng, tšobotsi e molemo ka ho khetheha bakeng sa ho felisa ho kuta lisele le ho hanyetsa ho phalla. Ho li etsa tse loketseng ho sebetsana le lisampole tse thata-to-pipette joalo ka mela e robehileng ea lisele, DNA ea genomic, hepatocytes, hybridomas, le lino tse ling tse hlakileng haholo. Malebela ana a fokotsa matla a ho kuta ka mochini, ho thibela ho arohana ha lisele le ho netefatsa hore lisele li sebetsa hantle le ho sebetsa hantle.

6. Litlhahiso tsa Robotic Pipette

Litlhahiso tsa pipette ea lirobotoli etselitsoe ho sebelisoa le mefuta e fapaneng ea litsamaiso tsa ho sebetsana le mokelikeli le liroboto tsa liphaephe. Litlhahiso tsena li netefatsa hore li lumellana le lihlahisoa (Hamilton, Beckman, Agilent, Tecan, joalo-joalo) ho li-automation tsa laboratori, ho ntlafatsa ho nepahala le ho sebetsa hantle lits'ebetsong tse phahameng. Litlhahiso tsa liroboto li laoloa tlas'a mamello e matla ha li bapisoa le malebela a pipette. Malebela ana a liroboto tse ikemetseng a netefatsa ho nepahala ho holimo, ho nepahala le ho sebetsa hantle lits'ebetsong tsa ts'ebeliso e phahameng ho pholletsa le mafapha a fapaneng, ho kenyeletsoa genomics, proteomics, le lipatlisiso tsa meriana.

Mohlala:

Litlhahiso tse tsamaisang pipetteke malebela a khethehileng a sebelisoang lits'ebetsong tsa liphaephe tse ikemetseng tse etselitsoeng ho fokotsa ho bokellana ha tefiso ea electrostatic nakong ea ho sebetsana le mokelikeli. Malebela ana a bohlokoa bakeng sa lits'ebetso moo tšitiso ea electrostatic e ka amang bots'epehi ba sampole kapa ho nepahala ha litsamaiso tse sebetsanang le mokelikeli o ikemetseng.

7. Litlhahiso tse khethehileng tsa Pipette

Lisebelisoa tse ling li hloka meralo e ikhethang ea malebela a pipette bakeng sa mesebetsi e itseng.

Mehlala:

Litlhahiso tsa PCR:Malebela a etselitsoeng ho sebelisoa lits'ebetsong tsa polymerase chain reaction (PCR) ho thibela tšilafalo ho tsoa ho DNA e matlafalitsoeng.
Litlhahiso tsa Cryogenic:E etselitsoe ho sebelisoa ka mocheso o tlase haholo, 'me hangata e tla ka moaho o matla, o tšoarellang ho sebetsana le lisampole tse leqhoa.

Qetello

Khetho ea malebela a pipette e itšetlehile ka mofuta oa teko le mofuta oa pipette e sebelisoang. Ebang ke bakeng sa ho sebetsana le mokelikeli ka kakaretso, ho thibela tšilafalo, kapa ho sebetsa ka lisampole tse bobebe kapa tse turang, ho utloisisa mefuta le litšobotsi tsa malebela a pipette ho netefatsa phetiso e nepahetseng le e sebetsang ea mokelikeli ka laboratoring. Kamehla khetha keletso e nepahetseng ea pipette bakeng sa litlhoko tsa hau tse khethehileng tsa lipatlisiso ho netefatsa liphello tse nepahetseng le ho tšepahala.