Lisebelisoa tse Tloaelehileng tsa ELISA Plates

E le sesebelisoa sa liteko, sebopeho sa mantlha saELISA poleitike letoto la li-microplates tse nang le lisebelisoa tse tiileng tsa mohato (joaloka liprotheine le li-antibodies). Tšebelisong ea poleiti ea ELISA, sampole e tla lekoa e tla itšoara ka molek'hule e itseng e ngotsoeng ke enzyme, ebe phetoho e bonahalang ea 'mala e tla hlahisoa ka ho eketsa substrate ea matrix,' me litaba kapa mosebetsi oa molek'hule o reretsoeng o tla lekanngoa. kapa e hlahlojwa ka ho lemoha letshwao la ho monya kapa la fluorescence. Tse latelang ke ts'ebeliso e tloaelehileng ea lipoleiti tsa ELISA libakeng tse fapaneng:

1. Tlhahlobo ea palo ea liprotheine: Lipoleiti tsa ELISA li ka sebelisoa ho lekanya mahloriso le mosebetsi oa liprotheine ka lisampole tsa likokoana-hloko tse kang serum le li-cell supernatants, ho fana ka lisebelisoa tse matla bakeng sa ho lemoha matšoao a hlahala, li-antibodies tsa kokoana-hloko ea lefu la sebete, matšoao a kotsi a myocardial, joalo-joalo. le ho thusa lingaka tlhahlobong ea pele le tlhahlobo ea mafu.

2. Tlhokomelo ea cytokine: Lipatlisisong tsa immunology,ELISA lipoleitie ka lekanya maemo a cytokine ho li-supernatant tsa cell culture kapa maro a lisele, tse thusang ho utloisisa mekhoa ea likokoana-hloko tse kang likarabo tsa 'mele oa ho itšireletsa mafung le likarabo tsa ho ruruha,' me ke tsa bohlokoa bo boholo bakeng sa nts'etsopele ea phekolo e ncha le lithethefatsi.

3. Lipatlisiso tsa Nucleic acid: Ka lipoleiti tsa ELISA, bo-rasaense ba ka lemoha le ho hlahloba litaba le mosebetsi oa DNA kapa RNA, ba fana ka tšehetso ea data bakeng sa lipatlisiso tsa baeloji ea limolek'hule tse kang gene expression le gene regulation, le ho tsoela pele ho khothalletsa nts'etsopele ea likarolo tse kang gene therapy. le ho lokisa liphatsa tsa lefutso.

4. Lipatlisiso tsa ts'ebetso ea enzyme: Lipoleiti tsa ELISA li ka lekanya ka nepo mosebetsi oa enzyme, tsa thusa bafuputsi ho utloisisa mosebetsi le mokhoa oa taolo oa li-enzyme linthong tse phelang, le ho fana ka litšupiso tsa bohlokoa bakeng sa lipatlisiso tsa boenjiniere ba enzyme, boenjiniere ba metabolic le likarolo tse ling.

5. Patlisiso ea likamano tsa limolek'hule:ELISA lipoleitie ka sebelisoa eseng feela ho lekanya litaba tsa limolek'hule, empa hape le ho ithuta tšebelisano pakeng tsa limolek'hule. Ka ho kopanya theknoloji e kang holim'a plasmon resonance le fluorescence resonance energy transfer, mokhoa o tlamang le oa ho arohana pakeng tsa limolek'hule o ka hlahlojoa ka nako ea sebele, ho fana ka maikutlo a macha le mekhoa ea ho etsa lithethefatsi, ho sebelisana ha protheine le lipatlisiso tse ling.