Ho Fihla ho Ntjha | THEKISO | Black Elisa Plates

Maemong a fapaneng a mahlale a bophelo, boikemisetso bo nakong, bo sebetsang hantle le ba moruo le palo ea li-antigen kapa li-antibodies tse teng sampoleng ke karolo ea bohlokoa.

Tekanyetso ea immunosorbent e amanang le enzyme (ELISA) e ipakile e le sesebelisoa sa bohlokoa sa lipatlisiso le tlhahlobo bakeng sa ho lekanya li-antibodies kapa li-antigen ka lisampole tsa likokoana-hloko ka ho kenngoa ha li-antigen kapa li-antibodies tse tsejoang holim'a mochine o tiileng oa mohato, o lumellang enzyme. haholo-holo HRP) -e ngotsoeng ka li-antigen-antibody reactions sebakeng se tiileng-mohato. Mokhoa ona o ka sebelisoa ho lemoha li-antigen tse kholo tsa molek'hule le li-antibodies tse khethehileng, joalo-joalo E na le melemo ea ho potlaka, ho utloahala, ho le bonolo, 'me ho bonolo ho e tsamaisa. Leha ho le joalo, kutloisiso le mefuta e matla ea ho lemoha ELISA e fokolloa haholo ke mefokolo ea mokhoa oa ho monya leseli ka lebaka la tšusumetso e kholo ea maemo a ka ntle ho phetoho ea 'mala oa tharollo le mefuta e tlaase e sebetsang ea linear ea boleng ba OD.

Theknoloji ea DELFIA ---- ke feela ho nkela enzyme HRP sebaka ka lanthanide chelate (Eu, Sm, Tb, Dy) e ngotseng lesole la 'mele la ho lemoha litlhahlobong tsa setso tsa ELISA. Li-lanthanides tse sebelisoang ho DELFIA ke sehlopha se ikhethileng sa likarolo tsa fluorescent, tse behang tlhokahalo ea lisebelisoa tsa liteko --- lipoleiti tsa Elisa. Li-Lanthanides li na le linako tsa bophelo tsa fluorescence tsa microseconds kapa esita le li-milliseconds, tseo ha li kopane le ho lemoha nako e rarollotsoeng haholo li fokotsang tšitiso ea mokokotlo oa autofluorescence, 'me phetoho ea bona ea Strokes' e pharaletseng e ntlafatsa haholo kutloisiso ea tlhahlobo.

Bongata bo boholo ba ELISA bo khetha poleiti e hlakileng ea enzyme e le sejari le sets'oants'o, empa leseli le hlahisoang karabelong ea luminescence ke isotropic, leseli le ke ke la qhalakanngoa feela ho tloha ntlheng e otlolohileng, empa hape le hasane ho tloha ka lehlakoreng le otlolohileng, 'me feta habonolo lekhalo pakeng tsa masoba a sa tšoaneng a pepeneneng enzyme labeling poleiti le lebota la holes.Neighboring masoba sebelisana le mong ho e mong le ho ama liphello tsa liteko.

White Elisa Plates e ka sebelisoa bakeng sa ho lemoha leseli le fokolang 'me hangata e sebelisoa bakeng sa chemiluminescence e akaretsang le nts'etsopele ea mebala ea substrate (mohlala, tlhahlobo ea liphatsa tsa lefutso tsa moqolotsi oa litaba oa luciferase).

Black White Elisa Plates e na le lets'oao le fokolang ho feta lipoleiti tse tšoaeang enzyme e tšoeu ka lebaka la ho monya ha tsona, 'me hangata li sebelisoa ho bona khanya e matla, joalo ka ho lemoha ha fluorescence.

Melemo ea Cotaus®Elisa Plates

● Tlamahano e phahameng

Lipoleiti tsa Cotaus®Elisa tse nang le li-tube tse ntšo li entsoe ka thepa e sa itšetleheng ka fluorescent, bokaholimo bo sebelisitsoe ho ntlafatsa haholo bokhoni ba eona ba ho tlama protheine, bo ka fihlang ho 500ng IgG/cm2, mme boima ba limolek'hule tsa protheine e kholo ke> 10kD. .

● Fluorescence e tlase e felisa mathata a bakoang ke ho se tobane le maikutlo a itseng.

li-tubs tse ntšo li ka felisa tšitiso e fokolang ea mokokotlo oa fluorescence hobane e tla ba le khanya ea eona.

● Moralo o ka arohang

Moralo o ka senyehang oa foreimi ea poleiti e tšoeu ea enzyme le li-slats tsa enzyme e ntšo li bonolo bakeng sa ts'ebetso. Ela hloko ketso ea ho qhaqha, u se ke ua qobella ho senya ka lehlakoreng le leng, ho seng joalo ho tla ba bonolo ho senya.

Sehlopha sa Lihlahisoa

Bakeng sa lintlha tse ling tsa sehlahisoa, ka kopo ikutloe u lokolohile ho ikopanya le rona